Lysinibacillus manganicus sp. nov., isolated from manganese mining soil.

A Gram-stain-positive, aerobic, motile, rod-shaped bacterium, designated strain Mn1-7(T), was isolated from manganese mining soil in Tianjin, China. The closest phylogenetic relatives were Lysinibacillus massiliensis CCUG 49529(T) (97.2 % 16S rRNA gene sequence similarity), L. xylanilyticus XDB9(T) (96.7 %), L. sinduriensis JCM 15800(T) (96.2 %), L. odysseyi NBRC 100172(T) (95.9 %) and L. boronitolerans NBRC 103108(T) (95.4 %) (the type species of the genus). DNA-DNA hybridization values for strain Mn1-7(T) with the type strains of L. massiliensis and L. sinduriensis were 24.9 and 27.7 %, respectively. The genomic DNA G+C content was 38.4 mol%. The major menaquinone was MK-7 and the major fatty acids were iso-C15 : 0, iso-C16 : 0 and iso-C14 : 0. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The cell-wall peptidoglycan was type A4α (L-Lys-D-Asp), and the predominant cell-wall sugar was xylose. DNA-DNA hybridization results and comparison of phenotypic and chemotaxonomic characters between strain Mn1-7(T) and the phylogenetically most closely related strains revealed that the isolate represents a novel species of the genus Lysinibacillus, for which the name Lysinibacillus manganicus sp. nov. is proposed. The type strain is Mn1-7(T) ( = DSM 26584(T) = CCTCC AB 2012916(T)).